Effects of Qingre Antai Gel on ANXA1/FPR2 Axis and Cell Apoptosis in Gingival Tissue of Rats with Pregnancy Gingivitis

Corresponding author: CHEN ShiJuan, zgrao@126.com

DOI: 10.12201/bmr.202610.00028

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  Abstract: Objective: To investigate the intervention effect and mechanism of self-made Qingre Antai gel on experimental pregnancy gingivitis rats based on ANXA1/FPR2 axis. Methods: The pregnancy gingivitis model in SD rats was established by suture ligation method. The rats were randomly divided into four groups: blank control group, model group, positive control group (normal saline irrigation), and Chinese medicine group (Qingre Antai gel). After 7 days of intervention, the gingival status of rats was observed. The gene expressions of ANXA1 and FPR2 in gingival tissue were detected by qPCR, the protein expressions of ANXA1 and FPR2 were detected by Western blot, and the level of gingival cell apoptosis was observed by TUNEL fluorescence staining. Results: Compared with the blank group, the model group showed obvious gingival redness and swelling, the mRNA expression levels of ANXA1 and FPR2 increased to 2.64 and 2.06 times of the blank group respectively, the protein expression increased compensatorily, and the apoptotic cells in gingiva increased significantly. The positive control group could alleviate gingival redness and swelling, the mRNA expression levels of ANXA1 and FPR2 increased significantly to 3.62 and 3.00 times of the blank group respectively, the protein expression increased significantly, and the number of apoptotic cells was less than that in the model group. The Chinese medicine gel group could significantly alleviate gingival redness and swelling, and the mRNA expression levels of ANXA1 and FPR2 increased significantly to 5.31 and 4.51 times of the blank group respectively, the protein expression also increased significantly, and the number of apoptotic cells was less than that in the positive control group. The differences among groups were statistically significant (P<0.05). Conclusion: Qingre Antai gel can up-regulate the expressions of ANXA1 and FPR2 in gingival tissue of pregnancy gingivitis rats, reduce the level of tissue cell apoptosis, alleviate gingival tissue redness, swelling and damage, and relieve local damage of pregnancy gingivitis, providing experimental basis for safe local medication during pregnancy.

  Key words: Pregnancy gingivitis; Qingre Antai gel; ANXA1; FPR2; Cell apoptosis

  Submit time: 13 October 2026

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
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