Correlation between the Expression of Peripheral Blood miR-145-5p and miR-223 and Treatment Response and Prognosis in Patients with Chronic Obstructive Pulmonary Disease Complicated with Pulmonary Tuberculosis

Corresponding author: Chen Zhen, w15722907113@163.com

DOI: 10.12201/bmr.202604.00163

•  

  Abstract: Objective? To investigate the correlation between the expression levels of peripheral blood microRNA-145-5p (miR-145-5p) and microRNA-223 (miR-223) and treatment response and prognosis in patients with chronic obstructive pulmonary disease (COPD) complicated with pulmonary tuberculosis (PTB).Methods? A total of 180 patients with COPD complicated with PTB treated in this hospital from March 2022 to March 2024 were enrolled as the study group. Meanwhile, 60 COPD patients, 60 PTB patients and 60 healthy subjects during the same period were selected as the control cohort. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to detect the baseline expression levels of peripheral blood miR-145-5p and miR-223 in all subjects, and the above indicators were dynamically measured in the study group at 3 and 6 months of treatment. The expression levels of miR-145-5p and miR-223 were compared among patients with different treatment responses and prognostic outcomes in the study group. Receiver operating characteristic (ROC) curve was used to analyze the predictive value of miR-145-5p and miR-223 expression levels for treatment response in patients with COPD complicated with PTB. Multivariate Logistic regression analysis was performed to explore the influencing factors for prognosis in these patients.Results? The baseline expression level of peripheral blood miR-145-5p in the study group was significantly lower than that in the COPD group, PTB group and healthy control group (P<0.05), while the expression level of miR-223 was significantly higher than that in the three groups above (P<0.05). After 6 months of treatment, 126 patients in the study group achieved effective treatment (effective group) and 54 had ineffective treatment (ineffective group). In the effective group, the expression level of miR-145-5p gradually increased and that of miR-223 gradually decreased at each time point after treatment (P<0.05), whereas no significant changes were found in the above indicators in the ineffective group (P>0.05). The area under the ROC curve (AUC) of baseline miR-145-5p combined with miR-223 for predicting treatment response was 0.842 (95% CI: 0.778~0.905), with a sensitivity of 88.1% and a specificity of 78.3%, which was superior to single indicator detection. During the 2-year follow-up, 28 patients in the study group experienced recurrence and 16 died. The poor prognosis group (recurrence or death) showed lower baseline miR-145-5p expression and higher miR-223 expression (P<0.05). Multivariate Logistic regression analysis revealed that low baseline expression of miR-145-5p, high baseline expression of miR-223, multi-lobe pulmonary tuberculosis lesion extent, elevated interleukin-6 (IL-6) and C-reactive protein (CRP) levels were independent risk factors for poor prognosis in patients with COPD complicated with PTB (P<0.05).Conclusion? Peripheral blood miR-145-5p is down-regulated and miR-223 is up-regulated in patients with COPD complicated with PTB. Combined detection of the two can serve as potential biomarkers for evaluating treatment response and prognosis of such patients, providing a reference for clinical individualized treatment and prognosis management.

  Key words: chronic obstructive pulmonary disease; pulmonary tuberculosis; microRNA-145-5p; microRNA-223; treatment response; prognosis

  Submit time: 27 April 2026

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
• html

• 图表

• Chen Cuiyan, Liang Huiying. Development of an Ensemble Screening Model for Chronic Obstructive Pulmonary Disease Using Multimodal Data. 2026. doi: 10.12201/bmr.202604.00086

  WU Junliang, TANG Jing, YANG Jing. Determinations of HBP, IL-6, and PCT for the Application in patients with Chronic obstructive pulmonary disease. 2026. doi: 10.12201/bmr.202604.00134

  LI LinHao, WANG Kun, LI Peng, XU XiaoChen, HAN Ping. Study on Chronic Obstructive Pulmonary Disease Complicated with Malnutrition Based on the TCM Theory of Child Consumes Qi of Mother. 2026. doi: 10.12201/bmr.202601.00090

  Hongying Ma. Advances in Exhaled Breath Condensate for Chronic Obstructive Pulmonary Disease. 2025. doi: 10.12201/bmr.202512.00038

  Zhou Jie², Xiao Wei², Sun Zengtao. Understanding the Mechanism and Therapeutic Strategies of the Gut-Lung Axis in Chronic Obstructive Pulmonary Disease from the Perspective of. 2026. doi: 10.12201/bmr.202601.00005

  Liao Siqing, Zhou Xia. Management of chronic pulmonary aspergillosis with hemoptysis. 2025. doi: 10.12201/bmr.202512.00043

  liushunlin. The expression of CPNE1 and ZWILCH in the progression of lung adenocarcinoma and their correlation with prognosis. 2024. doi: 10.12201/bmr.202406.00030

  Jing ZHAO, Junxia HUANG, Xiuhua TANG, Hong WANG. The role of HOXA11-AS/miR-149-3p pathway in the malignant phenotype of lung squamous cell carcinoma. 2022. doi: 10.12201/bmr.202204.00005

  张佳倩, Qi Ruonan, Ma Hongying, Liu Qingbing, Lv Dan. . 2025. doi: 10.12201/bmr.202511.00035

  XIANG Hongying, LING Yingchun. Analysis of the predictive value of quantitative parameters of electroencephalogram and serum miR-146a-5p for cognitive impairment in Alzheimers disease patients. 2024. doi: 10.12201/bmr.202412.00027

• ID	Submit time	Number	Download
  1	2026-04-10	10.12201/bmr.202604.00163V1	Download

• Public  Anonymous  To author only Submit Comments