Shenduning alleviates inflammation and oxidative stress in a rat model with acute kidney injury induced by contrast agents by regulating the Nrf2/HO-1 signaling pathwayJiangjiangang  yuanzheng  liangliguo zhouhuadong

DOI: 10.12201/bmr.202610.00038

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  Abstract: Objective To explore the effect of Shenduning pretreatment on inflammation and oxidative stress in contrast agent-induced acute kidney injury (CI-AKI) model rats by regulating the Nrf2/HO-1 signaling pathway. Methods Forty male SD rats were randomly divided into the control group (CON group), the model group (MOD group), the Shenduning group (SDN), and the Shenduning +ML-385 group (ML group), with 10 rats in each group. One week before modeling, the SDN group was given a decoction of Shenduning by gavage, while the ML group was given an intraperitoneal injection of ML-385 (a specific inhibitor of Nrf2) on the basis of the Shenduning group. Compare the differences in the levels of serum renal function (SCr, BUN), inflammatory factors (IL-6, TNF-α, IL-1β), pathological changes of renal tissue, oxidative stress in renal tissue (GSH, SOD, MDA), ROS and the number of apoptotic cells among each group of rats. The expression levels of Nrf2/HO-1 pathway proteins in renal tissue were detected by Western blot. Results Compared with MOD group, the serum renal function of rats in the SDN group was better, the levels of inflammatory factors were lower, the pathological changes of renal tissue were milder, the level of oxidative stress in renal tissue was lower, the level of ROS was lower, the number of apoptotic cells was smaller, and the expression of Nrf2/HO-1 was higher(P＜0.05). Compared with SDN group, the serum renal function of rats in the ML group was worse, the levels of inflammatory factors were higher, the pathological changes of renal tissue were more severe, the level of oxidative stress in renal tissue was higher, the level of ROS was higher, the number of apoptotic cells was greater, and the expression of Nrf2/HO-1 was lower(P＜0.05). Conclusion Pretreatment with Shenduning may effectively improve renal function in CI-AKI model rats, reduce the levels of inflammatory factors, oxidative stress, ROS and the number of apoptotic renal cells, and increase the expression of the Nrf2/HO-1 signaling pathway.

  Key words: contrast-induced acute kidney injury; shenduning; Nrf2/HO-1; oxidative stress; inflammatory factors

  Submit time: 17 October 2026

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
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