通讯作者: 蒋健刚, jiangjiangang2020@163.com

DOI：10.12201/bmr.202610.00038

Shenduning alleviates inflammation and oxidative stress in a rat model with acute kidney injury induced by contrast agents by regulating the Nrf2/HO-1 signaling pathwayJiangjiangang  yuanzheng  liangliguo zhouhuadong

• 摘要：目的 探索肾毒宁预处理通过调节Nrf2/HO-1信号通路对对比剂诱导急性肾损伤（CI-AKI）模型大鼠炎症和氧化应激的影响。方法 将40只SD雄性大鼠随机分为对照组（CON组）、模型组（MOD组）、肾毒宁组（SDN）和肾毒宁+ML-385组（ML组），每组各10只。SDN组在造模前1周用肾毒宁水煎剂灌胃，ML组在肾毒宁组的基础上增加ML-385（Nrf2特异性抑制剂）腹腔注射。比较各组大鼠血清肾功能（SCr、BUN）、炎症因子（IL-6、TNF-α、IL-1β）、肾组织病理变化、肾组织氧化应激（GSH、SOD、MDA）、ROS及凋亡细胞数等水平的差异，免疫印记检测肾组织Nrf2/HO-1的通路蛋白的表达水平。结果 与MOD组比较，SDN组大鼠血清肾功能更好，炎症因子水平更低，肾组织病理变化更轻，肾组织氧化应激水平更低，ROS水平更低，凋亡细胞数更少，Nrf2/HO-1表达更高（P＜0.05）。与SDN组比较，ML组大鼠血清肾功能更差，炎症因子水平更高，肾组织病理变化更重，肾组织氧化应激水平更高，ROS水平更高，凋亡细胞数更多，Nrf2/HO-1表达更低（P＜0.05）。结论 肾毒宁预处理可以有效改善CI-AKI模型大鼠肾功能，降低炎症因子、氧化应激水平、ROS及肾细胞凋亡数，提高Nrf2/HO-1信号通路的表达。

  关键词： 对比剂诱导急性肾损伤; 肾毒宁; Nrf2/HO-1; 氧化应激; 炎症因子

   

  Abstract: Objective To explore the effect of Shenduning pretreatment on inflammation and oxidative stress in contrast agent-induced acute kidney injury (CI-AKI) model rats by regulating the Nrf2/HO-1 signaling pathway. Methods Forty male SD rats were randomly divided into the control group (CON group), the model group (MOD group), the Shenduning group (SDN), and the Shenduning +ML-385 group (ML group), with 10 rats in each group. One week before modeling, the SDN group was given a decoction of Shenduning by gavage, while the ML group was given an intraperitoneal injection of ML-385 (a specific inhibitor of Nrf2) on the basis of the Shenduning group. Compare the differences in the levels of serum renal function (SCr, BUN), inflammatory factors (IL-6, TNF-α, IL-1β), pathological changes of renal tissue, oxidative stress in renal tissue (GSH, SOD, MDA), ROS and the number of apoptotic cells among each group of rats. The expression levels of Nrf2/HO-1 pathway proteins in renal tissue were detected by Western blot. Results Compared with MOD group, the serum renal function of rats in the SDN group was better, the levels of inflammatory factors were lower, the pathological changes of renal tissue were milder, the level of oxidative stress in renal tissue was lower, the level of ROS was lower, the number of apoptotic cells was smaller, and the expression of Nrf2/HO-1 was higher(P＜0.05). Compared with SDN group, the serum renal function of rats in the ML group was worse, the levels of inflammatory factors were higher, the pathological changes of renal tissue were more severe, the level of oxidative stress in renal tissue was higher, the level of ROS was higher, the number of apoptotic cells was greater, and the expression of Nrf2/HO-1 was lower(P＜0.05). Conclusion Pretreatment with Shenduning may effectively improve renal function in CI-AKI model rats, reduce the levels of inflammatory factors, oxidative stress, ROS and the number of apoptotic renal cells, and increase the expression of the Nrf2/HO-1 signaling pathway.

  Key words: contrast-induced acute kidney injury; shenduning; Nrf2/HO-1; oxidative stress; inflammatory factors

  提交时间：2026-10-17

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• 序号	提交日期	编号	操作
  1	2026-09-06	10.12201/bmr.202610.00038V1	下载

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