Exploring the Mechanism of Chuangyang Ointmentin Treating diabetes Foot Based on Network Pharmacology and Molecular Docking

Corresponding author: XiangLiping, 1351637479@qq.com

DOI: 10.12201/bmr.202605.00063

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  Abstract: Objective: To thoroughly explore the potential mechanism of Wound Healing Ointment in treating Diabetic Foot Ulcer (DFU) using network pharmacology methods. Methods: Based on the TCMSP, BATMAN-TCM, and UniProt databases, active ingredients of Wound Healing Ointment and their corresponding targets were screened and integrated. Disease-related targets were obtained from the GeneCards, OMIM, and TTD databases to construct the DFU target dataset. The STRING database was used to build a protein-protein interaction (PPI) network, identify core targets, and perform GO functional annotation and KEGG pathway enrichment analysis via the DAVID database. Relevant results were visualized using the MicrobeCloud platform. Finally, molecular docking was employed to preliminarily validate the screening results. Results: A total of 88 active ingredients and 886 potential targets of Wound Healing Ointment were identified. Key intersecting targets were obtained after mapping with DFU disease targets. PPI network analysis revealed that proteins such as CASP3, BCL2, HIF1A, IL6, and AKT1 were located at the core of the network, suggesting their potential role in regulating and participating in biological processes such as responses to external stimuli, MAPK cascade activation, apoptosis, and proliferation. KEGG enrichment analysis further demonstrated that Wound Healing Ointment may treat DFU through synergistic effects involving multiple pathways and targets. Molecular docking results indicated that both core active ingredients and core targets exhibited favorable docking activity. Conclusion: This study preliminarily reveals that Wound Healing Ointment has a comprehensive mechanism of action characterized by multiple components, targets, and pathways in treating DFU, providing a scientific basis for elucidating its pharmacological effects and offering references for further research and clinical applications.

  Key words: Ulcer ointment; Diabetes foot ulcer; Network pharmacology; Molecular docking; mechanism of action

  Submit time: 18 May 2026

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
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