Investigating the Mechanism of Danhuang Powder in Ameliorating Diabetic Nephropathy: Network Pharmacology Prediction and Molecular Docking Verification.

Corresponding author: Zhao Sheng, 512037881@qq.com

DOI: 10.12201/bmr.202512.00029

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  Abstract: Objective: To elucidate the mechanisms by which Danhuang Powder (DHS) treats diabetic nephropathy (DN) using network pharmacology and molecular docking.Methods: Active compounds were retrieved from TCMSP and HERB; DN-related targets were obtained from GeneCards, TTD, and OMIM. Overlapping targets were identified (Venny), followed by PPI construction and topological analysis to determine core targets. GO and KEGG enrichment analyses characterized relevant biological processes and pathways. A herb–compound–disease–target network was built. Key compound–target interactions were validated by molecular docking (AutoDock Vina) and visualized in PyMOL.Results: 165 common targets were identified. Core targets included AKT1,TP53,STAT3 and NF-κB1. GO enrichment yielded 2,805 biological processes, 225 molecular functions and 81 cellular components; KEGG analysis identified 169 significant pathways. Molecular docking showed strong binding affinities between principal DHS compounds and core targets.Conclusion: DHS exerts therapeutic effects on DN via multi-component, multi-target and multi-pathway mechanisms, supporting its clinical application and further pharmacological investigation.

  Key words: Danhuang Powder; Diabetic Nephropathy; Network Pharmacology; Molecular Docking.

  Submit time: 10 December 2025

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
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