The role of HOXA11-AS/miR-149-3p pathway in the malignant phenotype of lung squamous cell carcinoma

Corresponding author: Hong WANG, wanghong56@163.com

DOI: 10.12201/bmr.202204.00005

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  Abstract: Objective To explore the mechanism of long non-coding RNA HOXA11-AS in the carcinogenesis of lung squamous cell carcinoma. Methods The expression of HOXA11-AS, miR-149-3p and SPT16 was detected by quantitative polymerase chain reaction (RT-qPCR). The cell viability was detected by MTT method. Double luciferase reporter gene was used to detect the interaction between miR-149-3p and HOXA11-AS or SPT16. Results In lung squamous cell carcinoma, the expression of HOXA11-AS and SPT16 increased, while the expression of miR-149-3p decreased. MiR-149-3p is the target of HOXA11-AS, while SPT16 is the target of miR-149-3p. HOXA11-AS knockout inhibited the proliferation of lung squamous cell carcinoma cells, while the use of miR-149-3p inhibitor could antagonize the inhibitory effect of HOXA11-AS knockdown on the proliferation of lung squamous cell carcinoma cells. In addition, up-regulation of SPT16 can weaken the anti-proliferation effect of si-HOXA11-AS-mediated lung squamous cell carcinoma cells. Conclusion The knockdown of HOXA11-AS may play an inhibitory role in lung squamous cell carcinoma by regulating the miR-149-3p/SPT16 axis.

  Key words: Lung squamous cell carcinoma; HOXA11-AS; miR-149-3p; competitive endogenous RNA

  Submit time: 20 April 2022

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
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