通讯作者: 刘冬冬, 497998186@qq.com

DOI：10.12201/bmr.202604.00065

Exploring the Effect and Preliminary Mechanism of Qingqi Huatan Pill on ALI Based on Network Pharmacology

• 摘要：目的 基于网络药理学与体外实验探讨清气化痰丸治疗急性肺损伤(acute lung injury,ALI)的作用机制。方法 利用TCMSP数据库筛选清气化痰丸的活性成分及作用靶点,通过GeneCards数据库获取ALI相关靶点,取交集得到潜在作用靶点。构建“中药-成分-靶点”网络与蛋白质相互作用(Protein-Protein Interaction Network,PPI)网络,进行GO功能与KEGG通路富集分析。采用脂多糖(Lipopolysaccharide,LPS)诱导BEAS-2B细胞建立ALI模型,设置对照组、模型组及清气化痰丸低、中、高剂量组,通过CCK-8法检测细胞活力,ELISA法检测炎症因子白介素-6(interleukin-6,IL-6)、 肿瘤坏死因子α(tumor necrosis factor α,TNF-α)水平,Western blot法检测p-PI3K、p-AKT蛋白表达。结果 筛选得到清气化痰丸活性成分92个,对应靶点208个,与ALI相关靶点(10885个)交集获得161个潜在作用靶点。PPI网络分析筛选出AKT1、IL6、TNF等核心靶点。KEGG富集分析显示PI3K-Akt信号通路为主要通路。体外实验表明,与模型组相比,清气化痰丸各剂量组细胞活力显著升高(P<0.05),IL-6 、TNF-α水平显著降低(P<0.05),p-PI3K、p-AKT蛋白表达显著降低(P<0.05)。结论 清气化痰丸可能通过抑制PI3K/AKT信号通路活化,减轻炎症反应,从而发挥对急性肺损伤的保护作用。

  关键词： 清气化痰丸; 急性肺损伤; 网络药理学; PI3K/AKT信号通路

   

  Abstract: Objective To investigate the mechanism of Qingqi Huatan Pill in the treatment of acute lung injury (ALI) based on network pharmacology and in vitro experiments. Methods The active components and corresponding targets of Qingqi Huatan Pill were screened using the TCMSP database, and ALI-related targets were obtained from the GeneCards database. The intersecting targets were identified as potential therapeutic targets. A “Chinese medicine-component-target” network and a protein-protein interaction (PPI) network were constructed, followed by GO function and KEGG pathway enrichment analyses. An ALI model was established using LPS-induced BEAS-2B cells. Cells were divided into control group, model group, and low-, medium-, and high-dose Qingqi Huatan Pill groups. Cell viability was assessed using CCK-8 assay, TNF-α and IL-6 levels were detected by ELISA, and the protein expression of p-PI3K and p-AKT was measured by Western blot. Results A total of 92 active components and 208 corresponding targets of Qingqi Huatan Pill were identified, and 161 overlapping targets were obtained with ALI-related targets (10885). Core targets such as AKT1, IL6, and TNF were identified through PPI network analysis. KEGG enrichment analysis indicated that the PI3K-Akt signaling pathway was the primary pathway involved. In vitro experiments showed that compared with the model group, cell viability was significantly increased (P<0.05), while the levels of TNF-α and IL-6 were significantly decreased (P<0.05) in all Qingqi Huatan Pill treatment groups. Furthermore, the protein expression levels of p-PI3K and p-AKT were significantly reduced (P<0.05). Conclusion Qingqi Huatan Pill may exert protective effects against acute lung injury by inhibiting the activation of the PI3K/AKT signaling pathway and alleviating inflammatory responses.

  Key words: Qingqi Huatan Pill; acute lung injury; network pharmacology; PI3K/AKT signaling pathway

  提交时间：2026-04-09

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• 序号	提交日期	编号	操作
  1	2026-03-26	10.12201/bmr.202604.00065V1	下载

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