Advances in the application of amplification-free CRISPRCas cascade signal amplification technology

Corresponding author: yaoyachao, yaoyc@gd2h.org.cn

DOI: 10.12201/bmr.202410.00045

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  Abstract: Rapid and accurate molecular diagnosis plays an important role in life science fields such as precision medicine, environmental monitoring, and food safety. CRISPR/Cas system based on clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) has become a very effective molecular diagnostic tool due to its advantages of high sensitivity, high specificity, convenience and rapidity. CRISPR/Cas system has become a very effective molecular diagnostic tool due to its advantages of high sensitivity, high specificity, convenience and speed. However, CRISPR/Cas system-based assays increase the target concentration through a pre-amplification step, which may also generate problems such as non-specific amplification, aerosol contamination, complex operation and long assay cycle. Therefore, various amplification-free detection strategies have been developed, but the low sensitivity of these amplification-free detection strategies cannot meet the diagnostic needs of most diseases. A series of studies have demonstrated that the cascade signal amplification strategy has a broad application prospect in improving the sensitivity of CRISPR/cas-based assays, and the amplification-free CRISPR/Cas cascade signal amplification technology in disease diagnosis is reviewed. The application of amplification-free CRISPR/Cas cascade signal amplification technology in disease diagnosis is reviewed, and the main strategies of amplification-free CRISPR/Cas cascade signal amplification technology are highlighted.

  Key words: CRISPR/Cas; amplification-free detection; ultrasensitive detection; signal amplification

  Submit time: 16 October 2024

  Copyright: The copyright holder for this preprint is the author/funder, who has granted biomedRxiv a license to display the preprint in perpetuity.
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• ID	Submit time	Number	Download
  1	2024-09-12	10.12201/bmr.202410.00045V1	Download

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