通讯作者: 欧和生▲, hs_ou01@163.com

DOI：10.12201/bmr.202503.00028

Effect of zhuang medicine Adinandra nitida on blood pressure of hypertensive rats induced by L-NAME.

• 摘要：目的 初步探讨壮药石崖茶对L-NAME诱导高血压大鼠的血压影响及可能分子机制。方法 采用L-NAME 诱导高血压大鼠模型,根据实验分组为空白组、模型组、卡托普利组、石崖茶高、中、低剂量组。连续灌胃给药4周,每周测量大鼠血压1 次,末次给药1 h 后测量血压。腹腔注射麻醉,取材,采集血液样本。采用苏木精-伊红(Hematoxylin Eosin,HE)染色观察大鼠胸主动脉组织的病理变化,Western blotting检测大鼠胸主动脉内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)蛋白表达水平,酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)检测大鼠血浆 eNOS 含量,一步还原法检测大鼠血浆中一氧化氮(Nitric oxide,NO)含量。结果 与模型组比较,石崖茶高、中剂量组与卡托普利组大鼠收缩压和舒张压均显著降低(P＜0.05 或P＜0.01),心率变化无统计学意义(P> 0.05)。石崖茶灌胃4周后, L-NAME诱导高血压大鼠的血管组织损伤得到了明显改善,大鼠胸主动脉 eNOS蛋白表达明显增加(P<0.01),大鼠血浆中eNOS含量、NO含量显著增加(P<0.01)。结论 壮药石崖茶对L-NAME诱导高血压大鼠有降压作用,其可能与eNOS基因的表达和NO的调节有关。

  关键词： 石崖茶; L-NAME; 高血压; 内皮型一氧化氮合酶; 一氧化氮

   

  Abstract: Objective To investigate the effect of zhuang medicine Adinandra nitida on blood pressure of hypertensive rats induced by L-NAME and its possible molecular mechanism. Methods L-NAME was used to induce a hypertension rat model. According to the experimental group, they were divided into blank group, model group, Captopril group, and high, medium, and low dose groups of Shiya tea.and the experimental groups were divided into blank group, model group, Captopril group, and high, medium, and low dose groups of Adinandra nitida. The rats were continuous gavaged for 4 weeks, and the blood pressures were measured once a week. The last blood pressure was measured 1 hour after the last administration. Rats were anesthetized by intraperitoneal injection, tissues and blood samples were collected. Pathological changes of the rat thoracic aortic tissue were observed by hematoxylin Eosin (HE) staining, the protein expression level of endothelial nitric oxide synthase (eNOS) in rat thoracic aorta was detected by western blotting, the content of eNOS in rat plasma was measured by enzyme linked immunosorbent assay (ELISA) ,and the nitric oxide (NO) content in rat plasma was detected by One step reduction method. Results Compared with the model group, the high and medium dose groups of Adinandra nitida and the Captopril group of rats showed significant reductions in systolic blood pressure and diastolic blood pressure (P< 0.05 or P< 0.01), while there was no statistically significant change in heart rate (P>0.05). After 4 weeks of oral administration with Adinandra nitida, the damage of the vascular tissues of hypertensive rats induced by L-NAME has been improved, the expression of eNOS protein in thoracic aortic blood was significantly increased (P<0.01), and the contents of eNOS and NO in rat plasma were significantly increased (P<0.01). Conclusion Zhuang medicine Adinandra nitida has a hypotensive effect on L-NAME induced hypertensive rats, which may be related to the regulation of eNOS expression.

  Key words: Adinandra nitida; L-NAME; Hypertension; Endothelial nitric oxide synthase; Nitric oxide

  提交时间：2025-03-11

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• 序号	提交日期	编号	操作
  1	2025-02-15	bmr.202503.00028V1	下载

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